Journal: European Journal of Medicinal Chemistry Reports

Article Title: Kolaviron ameliorates chronic colitis induced by prolonged oral administration of Dextran Sulphate Sodium in Balb/c mice

doi: 10.1016/j.ejmcr.2022.100071

Figure Lengend Snippet: Fig. 3. Effect of Kolaviron (KOL) on pro-inflammatory proteins in Colitis Chronicity. Tumor Necrosis Factor Alpha (TNF-α) (A) Interleukin-1β (IL-1 β) (B) Inducible Nitric Oxide Synthase (iNOS) (C) Cyclooxygenase-2 (COX-2) (D). Each bar represents mean SD of ten mice. a: Values differ significantly from control (p < 0.05). b: Values differ significantly from KOL þ DSS (p < 0.05).

Article Snippet: Anti-iNOS and Anti-COX-2 mice monoclonal antibodies, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β), ELISA kits and were purchased from CUSABIO Life Science Inc. (Wuhan, China).

Techniques: Control

Journal: Neural Regeneration Research

Article Title: Inflammatory response and neuronal necrosis in rats with cerebral ischemia

doi: 10.4103/1673-5374.143419

Figure Lengend Snippet: Expression of intercellular cell adhesion molecule-1 (ICAM-1), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in serum of rats after middle cerebral artery occlusion. (A, C, D) Expression of ICAM-1, TNF-α and IL-1β in serum of rats. Data are expressed as the mean ± SD. One-way analysis of variance was used to evaluate differences between groups. Paired t -test was applied for intergroup comparison. * P < 0.05, ** P < 0.01, vs . control and sham surgery groups; # P < 0.05, ## P < 0.01, vs . other time points. (B) Correlation between ICAM-1 concentration and permeability of the blood-brain barrier ( r = 0.716, P = 0.002). (E) Correlation between TNF-á and IL-1â expression ( r = 0.755, P = 0.001). Intergroup Pearson correlation analysis was conducted. h: Hour(s); d: day(s).

Article Snippet: Briefly, 100 μL standard or sample were added to each well and incubated with mouse anti-rat intercellular cell adhesion molecule-1 monoclonal antibody (1:100; Uscn Life Science Inc., Wuhan, Hubei Province, China), mouse anti-rat tumor necrosis factor-alpha monoclonal antibody (1:100; Uscn Life Science Inc.) or mouse anti-rat interleukin-1 beta monoclonal antibody (1:100; Uscn Life Science Inc.) for 2 hours at 37°C.

Techniques: Expressing, Concentration Assay, Permeability

Journal: Emerging Microbes & Infections

Article Title: Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates

doi: 10.1080/22221751.2021.1931466

Figure Lengend Snippet: Specific humoral and cellular immune response of C57BL/6 and BALB/c mice to prime-boost immunization with Sad23L-nCoV-S and Ad49L-nCoV-S vaccines. (A) C57BL/6 and BALB/c mice ( n = 5/group) were prime immunized with a dose of 10 9 PFU Sad23L-nCoV-S vaccine and boosted with a dose of 10 9 PFU Ad49L-nCoV-S vaccine at 4 week interval. Sera and splenocytes were collected from vaccinated or control mice for measurement of antibody and T-cell responses 4 weeks after both prime only and boosting immunizations. (B–C) Anti-S-BAb and RBD-BAb titers determined by ELISA. (D–E) NAb titers measured by sVNT and pVNT. (F) IFN-γ secreting T-cell response (SFCs/million cells) to S peptides, S or RBD protein measured by ELISpot. (G) Frequency of IFN-γ or TNF-α expressing CD4 + and CD8 + T-cell response to S peptides determined by ICS. Data are shown as a mean ± SEM. P values are analysed with one-way ANOVA and two-tailed t test. Statistically significant differences are shown with asterisks (*, P < 0.05; **, P < 0.01 and ***, P < 0.001).

Article Snippet: Cells were fixed with IC fixation buffer, permeabilized with permeabilization buffer (BD) and stained with anti-mouse or anti-monkey interferon-γ (IFN-γ), interleukin-2 (IL-2), and tumour necrosis factor α (TNF-α) (BD).

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Expressing, Two Tailed Test

Journal: BMC Ophthalmology

Article Title: Retinal upregulation of inflammatory and proangiogenic markers in a model of neonatal diabetic rats fed on a high-fat-diet

doi: 10.1186/1471-2415-13-14

Figure Lengend Snippet: TNF-α retinal expression. The immunohistochemical expression of TNF-α was found to be upregulated in vessels of the inner retina of the diabetic group fed on a HFD (arrows) compared to diabetic group on a normal diet and controls. Barr 10 μm. Western blot confirms the higher expression in the diabetic group on a HFD. (***p < 0.0005).

Article Snippet: The following primary antibodies were used for immunohistochemical analyses: The anti-mouse polyclonal antibody against vascular endothelial growth factor (VEGF) (1:500) (Santa Cruz Biotechnology Inc. 2145 Delaware Avenue Santa Cruz, CA, USA); the anti-mouse polyclonal antibody against receptor advanced-glycation end products (RAGE) (1:300) (AnaSpec Inc, San Jose, CA, USA); the anti-mouse polyclonal antibody against 5-lipoxygenase (5-LO) (1:500) (Assay biotechnology company, San Francisco, CA, USA) and the anti-mouse polyclonal antibody against tumor necrosis factor-alpha (TNF-α) (1:100) (Assay Biotechnology Company, San Francisco, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Western Blot